Distinguishing DNA modifications
Cytosine modification enables fine tuning of gene expression and chromatin remodeling, and functions not only in cell differentiation and stem cell dynamics, but can contribute to neurodegenerative diseases and cancer.
Increasing evidence suggests that the different cytosine modifications such as 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC) are functionally distinct, and should be studied as such. However, traditional bisulfite sequencing cannot distinguish between these modifications, and yields a combined result of unknown proportions.
To address this problem, Cambridge Epigenetix have developed TrueMethyl©, which enables accurate quantification of 5mC and 5hmC in parallel at single-base resolution.
TrueMethyl is based upon the oxidative bisulfite sequencing (oxBS-Seq) methodology developed by Prof. Shankar Balasubramanian, who previously co-invented the Solexa / Illumina sequencing platform.